Product Overview:
Easy to handle PEPperCHIP® microarrays allow the profiling of immune responses in blood sera linked with infection, immunization, autoimmune diseases or cancer in a uniquely flexible and cost-effective manner. The PEPperCHIP® Coronavirus HCoV-EMC/2012 (Acute Respiratory Distress Syndrome) contains 3,819 15 aa peptides with a peptide-peptide overlap of 12 (Replicase polyprotein 1ab) and 13 amino acids (all other proteins). The C- and N-termini of all proteins were elongated by neutral GSGSGSG linkers to avoid truncated peptides.. Each slide contains one array and is compatible with a 3/1-well PEPperCHIP® Incubation Tray (PPC.004.001). Routinely implemented HA and Flag control peptides can be stained with antibodies provided in the PEPperCHIP® Staining Kit (PPC.002.001 and PPC.003.001). The PEPperCHIP® microarrays are compatible with most microarray scanners like the GenePix, Agilent and Innopsys readers or the Li-COR Odyssey Imaging System. For software-assisted image analysis of peptide microarray data we recommend PepSlide® Analyzer (PSA.001.001), but also provide GenePix Pro® support. Please download our SOPs for more information on chip handling, materials and methods.