Measure cGMP in lysed cells, plasma, saliva, urine or tissue

Measure to less than 1 fmol cGMP per sample - most sensitive kit available

The Direct Chemiluminescent Cyclic GMP (cGMP) Immunoassay kit is designed to quantitatively measure cGMP present in lysed cells, tissue plasma, urine, saliva and tissue culture media samples.

The kit is unique in that all samples and standards are diluted into an acidic Sample Diluent, containing special additives and designed to lyse cells, for measurement. AcGMP standard is provided to generate a standard curve for the assay. APlate Primer solution is added to all the wells and standards or samples in Sample Diluent are pipetted into the white microtiter plate. AcGMP-peroxidase conjugate is added to the standards and samples in the wells. The binding reac¬tion is initiated by the addition of an antibody to cGMP. After an overnight incubation at 4°C, the plate is washed and chemiluminescent substrate is added. The substrate reacts with the bound cGMP-peroxidase conjugate.

The intensity of the emitted light is detected in a microtiter plate reader capable of measuring luminescence. The concentration of cGMP in the sample is calculated, after making suitable correction for the dilutions. An optional Acetylation Format allows super low concentrations of cGMP to be measured.