Measure cAMP in lysed cells, plasma, saliva, urine or tissue

Measure to less than 1 fmol cAMP per sample - most sensitive kit available

High signals in 2 hours

The Cyclic AMP (cAMP) Chemiluminescent Immunoassay kit is designed to directly and quantitatively measure cAMP present in lysed cells, tissue plasma, urine, saliva and tissue culture media samples. The kit is unique in that all samples and standards are diluted into an acidic Sample Diluent, containing special additives and designed to lyse cells, for measurement. AcAMP stan¬dard is provided to generate a standard curve for the assay.

Primer solution is added to all the wells and standards or samples in Sample Diluent are pipetted into the white microtiter plate. AcAMP-peroxidase conjugate is added to the standards and samples in the wells. The binding reaction is initiated by the addition of an antibody to cAMP. After 2 hours, the plate is washed and chemiluminescent substrate is added. The substrate reacts with the bound cAMP-peroxidase conjugate. The intensity of the emitted light is detected in a microtiter plate reader capable of measuring luminescence. The concentration of cAMP in the sample is calculated, after making suit¬able correction for the dilutions. An optional Acetylation Format allows super low concentrations of cAMP to be measured