Product Overview:
Clonality: HIS.H8
Host Species; Mouse
Isotype: IgG2b
Immunogen: HHHHHH (6x His) synthetic peptide conjugated to KLH
Specificity: Recognizes the epitope of 6x His-tags encoded by many commercially available vectors, regardless of the tag's location in the fusion protein sequences (i.e.: reacts with N-terminal, C-terminal or internal 6x His-tags)
Purification: Protein A affinity chromatography from mouse ascites fluid
Applications: Dot, ELISA, IP, IS, WB
Sensitivity:WB (with ECL): 1-2ng of His-tagged fusion proteins