a-Ketoglutarate Dehydrogenase (KDH)...

a-Ketoglutarate Dehydrogenase (KDH) Assay Kit

Product Information

Product Overview:

a-Ketoglutarate dehydrogenase complex is a multienzyme complex present in the mitochondria. This enzyme catalyzes the oxidative decarboxylation of a-Ketoglutarate (KG) to succinyl-CoA with concomitant production of NADH which is proportional to the activity of the enzyme. This enzyme has been shown to be inactivated by oxidative stress. Therefore, the activity of this enzyme is a likely target of oxidative inactivation. The assay procedure uses 1-ml assay reaction volume, and requires quartz cuvettes of 1 ml capacity and a spectrophotometer with kinetic function with temperature control. The assay can be performed with isolated mitochondria or whole cell or tissue lysate.

Features:

Assay Kit includes:

1. Spectrophotometer equipped to perform enzyme kinetics with UV/VIS range with temperature control.
2. Quartz micro cuvettes capable of reading 1 ml reaction volume in a 1-cm light path.
3. Cell sonicator or disrupter.

Catalog#: KD-4022K
LID#: 224A
Discontinued
Order by Phone or Fax: 1.800.316.3081
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Specifications

Required Equipment1. Spectrophotometer equipped to perform enzyme kinetics with UV/VIS range with temperature control.
2. Quartz micro cuvettes capable of reading 1 ml reaction volume in a 1-cm light path.
3. Cell sonicator or disrupter.
MSDS AvailabilityYes, upon request.
Unit Size100 REACTIONS
Country of OriginUnited States of America
Safety StatementsFor research use only. Not for use in diagnostic procedures.
Sample AvailabilityNo

Shipping & Handling

Estimated Ship Time: 3 Business Days

Shipped In: Dry Ice

Hazardous Material: No

Storage

Storage Temperature: -20C

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