Total Glutathione Assay

Total Glutathione Assay

Product Information

Product Overview:

Glutathione is a major antioxidant protein in the cells and acts as a sink for toxic oxygen radicals. The assay utilizes glutathione reductase and NADPH as the source for reducing equivalents to reduce oxidized glutathione. Reduced glutathione reduces DTNB that produces a yellow color due to formation of 2 moles TNB. The concentration of assay is calculated using a standard curve.

Features:

Total Glutathione Assay includes:
1. Total Glutathione Assay Buffer
2. NADPH
3. DTNB (5,5’ –Dithiobis-2-Nitrobenzoic Acid)
4. Glutathione Reductase
5. Oxidized Glutathione
6. 0.5% NaHCO3

NOTE: 2, 3, 4 must be prepared daily.

Catalog#: TG-9022K
LID#: 223A
Quantity :
ESTIMATED SHIP DATE: 05/24/2018
Order by Phone or Fax: 1.800.316.3081
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Specifications

Required Equipment1. Cuvettes (1.5mL volume) plastic is okay.
2. Pipettes capable of measuring 1.0mL; 0.05mL; 0.02mL; and 0.10mL.
3. Spectrophotometer capable of absorption of 412nm and kinetics.
MSDS AvailabilityYes, upon request.
Unit Size100 REACTIONS
Country of OriginUnited States of America
Safety StatementsFor research use only. Not for use in diagnostic procedures.
Sample AvailabilityNo

Shipping & Handling

Estimated Ship Time: 3 Business Days

Shipped In: Dry Ice

Hazardous Material: No

Storage

Storage Temperature: -20C

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