Aconitase Assay Kit

Aconitase Assay Kit

Product Information

Product Overview:

The citric acid cycle enzyme aconitase is a member of a growing family of superoxide anion (O2.-) sensitive [4Fe—4S]-containing (de)- hydratases that has been implicated to be important sites of superoxide anion toxicity. The activity of aconitase is sensitive to inactivation by level of O2.-and is modulated by changes in O2.- levels in bacteria and mammalian cells. Thus, inactivation of aconitase can occur in response to oxygen radical generating compounds such as redox cycling agents, higher levels of oxygen or pathological conditions resulting in higher levels of O2.- . The assay is based on conversion of citrate to isocitrate by aconitase, and conversion of isocitrate to a-ketoglutarate by isocitric dehydrogenase. In this process, NADP+ is converted to NADPH, the absorbance of which is measured at 340 nm. The activity of aconitase is calculated as the rate of formation of NADPH and is expressed as milliunits of aconitase/mg protein.

Features:

Aconitase Assay kit includes:

1. Lysis Buffer
2. Aconitase Assay Buffer
3. Isocitric Dehydrogenase
4. NADP+

Catalog#: AC-2011K
LID#: 225A
Discontinued
Order by Phone or Fax: 1.800.316.3081
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Specifications

Required Equipment1. Spectrophotometer equipped to perform enzyme kinetics with UV/VIS range with temperature control.
2. Quartz semimicro cuvettes capable of holding 1 ml volume.
3. Pipettors for accurately measuring various solutions.
4. Dulbeco’s phosphate buffered saline (dPBS)
MSDS AvailabilityYes, upon request.
Unit Size100 REACTIONS
Country of OriginUnited States of America
Safety StatementsFor research use only. Not for use in diagnostic procedures.
Sample AvailabilityNo

Shipping & Handling

Estimated Ship Time: 3 Business Days

Shipped In: Dry Ice

Hazardous Material: No

Storage

Storage Temperature: -20C

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