MDR1 Antibody (C219)

MDR1 Antibody (C219)

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IHC-P: Use for Biotin-based detection systems. Sections should be pre-treated with an antigen unmasking solution. *Human liver (positive staining detected along luminal surfaces of bile canaliculi) or human colon (positive staining localized to luminal surface of secretaory epithelium) can be used as positive controls for IHC. **For optimal staining, the primary antibody should be incubated 60 minutes at room temperature. NOTE: Optimal dilutions/concentrations should be determined by the end user. Blocking of ATP hydrolysis by MDR1 confirmed by literature (PMID 1680871, 9016791, 10570132)

Catalog#: NB600-1036
LID#: 171735A
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Host SpeciesMouse
SpeciesHuman, Mouse, Rat, Canine, Primate
ControlsHuman liver (positive staining detected along luminal surfaces of bile canaliculi), human colon (positive staining localized to luminal surface of secretaory epithelium)
Entrez Human ID5243
LocalizationCell Membrane; Integral membrane protein
Preservation0.1% Sodium Azide
Species ReactivityCross-reacts with Human, Mouse, Dog, Primate and Rat. Expected to cross-react with wide range of Mammals due to highly conserved epitope sequence. Not yet tested in other species.
Swissprot Human IDP08183
Gene SymbolABCB1
ApplicationsWB, B/N, ICC/IF, IHC, IHC-P, IP
Species AbbreviationHu, Mu, Rt, Ca, Mk
Grade/PurityProtein G purified
ImmunogenSDS-solubilized plasma membranes of a multidrug resistant Chinese hamster ovary (CHO) cell line and a human cell line.
SpecificityThe specific binding of the clone C219 was stronger in epitope sequence VQAALD (N-Terminal fragment) than the epitope sequence VQEALD (C-terminal fragmant) (Georges E., et al., 1990).Analysis of p-glycoprotein expression in skeletal and cardiac muscle revealed that C219 cross reacts with a 200kDa protein that migrates in the same position as myosin. C219 has also been reported to detect the 185kDa c-erbB2 protein (Liu, et al 1997). Care should be taken in the interpretation of true positive P-glycoprotein expression when using myosin-containing tissues or tissues known to have p185 c-erbB2 expression.
EpitopeThis antibody recognizes an internal, highly conserved amino acid sequence: VQEALD and VQAALD, corresponding to the C-terminal and N-terminal regions, respectively, found in both MDR1 and MDR3 isoforms of P Glycoprotein.
Storage Buffer1% BSA, 0.01M PBS
DilutionBlocking/Neutralizing, Immunocytochemistry/Immunofluorescence 1:20, Immunohistochemistry 1:10-1:2000, Immunohistochemistry-Paraffin 1:50-1:500, Immunoprecipitation 1:200, Western Blot 1:200
Gene ID114609
Unit Size0.25ml
Safety StatementsThis product is for research use only and is not approved for use in humans or in clinical diagnosis.
Sample AvailabilityYes
SynonymABC20, ATP-binding cassette sub-family B member 1, ATP-binding cassette, sub-family B (MDR/TAP), member 1, CD243, CD243 antigen, colchicin sensitivity, CLCS, doxorubicin resistance, EC, EC 3.6.3, GP170, IBD13, MDR1MGC163296, multidrug resistance protein 1, PGY1P-GP, P-glycoprotein 1, P-gp

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Shipped In: Blue Ice

Hazardous Material: No


Storage Temperature: Store at 4C. Do not freeze.

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