MBD4 Antibody

MBD4 Antibody

Product Information

Product Overview:

MBD4, methyl-CpG binding domain protein 4 (MED1), is part of the mammalian DNA glycosylase superfamily. MBD4 may be involved in maintaining genome stability, epigenetics via DNA methylation, as well as carcinogenesis. MBD4 has an amino-proximal methyl-CpG binding domain and a C-terminal mismatch-specific glycosylase domain. This configuration is thought to help maintain genome stability (PMID: 21820404). Proteins with methylcytosine-binding domains act as transcriptional repressors via promoter methylation, as well as promote the gene-silencing effect of DNA methylation. A study of patients with primary immune thrombocytopenia has shown that a decrease in expression of MBD4 may be involved with the pathogenesis of the disease (PMID: 21377502). Additionally, MBD4 has been shown to be a human DNA repair protein that is possibly involved in DNA mismatch repair. Furthermore, a study investigating microsatellite instability found that the MBD4 codon 346 polymorphism may be a factor in colorectal cancer susceptibility in the Korean population (PMID: 19469655).

Application:

This MBD4 antibody is useful for Immunocytochemistry/Immunofluorescence and IHC-paraffin embedded sections. In ICC/IF, nuclear staining was observed in HeLa cells. In IHC-P, staining was observed in the nucleus of human breast cancer tissue. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. This antibody is not applicable for Western Blot.

Catalog#: NBP1-91189
LID#: 171795A
Quantity :
Order by Phone or Fax: 1.800.316.3081

Specifications

Host SpeciesRabbit
SpeciesHuman, Mouse (Negative)
Entrez Human ID8930
LocalizationNucleus.
Preservation0.05% Sodium Azide
Species ReactivityHuman. Does not react with mouse.
Swissprot Human IDO95243
Gene SymbolMBD4
ApplicationsICC/IF, IHC, IHC-P, WB (-)
Species AbbreviationHu, Mu(-)
Grade/PurityAffinity purified
ClonalityPolyclonal
ImmunogenA synthetic peptide made to an internal portion of the human MBD4 protein (between residues 200-250) [UniProt O95243]
Storage BufferPBS, 30% glycerol
DilutionImmunohistochemistry-Paraffin 1:400, Western Blot (Negative), Immunocytochemistry/Immunofluorescence 1:200, Immunohistochemistry 1:400
Unit Size0.1ml
Concentration1.11 mg/ml
Safety StatementsThis product is for research use only and is not approved for use in humans or in clinical diagnosis.
Sample AvailabilityYes
SynonymEC 3.2.2.-, G/T mismatch glycosylase, G/U mismatch glycosylase, MED1G/5-fluorouracil mismatch glycosylase with biphasic kinetics, methyl-CpG binding domain protein 4, methyl-CpG-binding domain protein 43,N(4)-ethenocytosine glycosylase, Methyl-CpG-binding endonuclease 1, Methyl-CpG-binding protein MBD4, Mismatch-specific DNA N-glycosylase, putative methyl-CpG binding protein

Shipping & Handling

Estimated Ship Time: 3 Business Days

Shipped In: Blue Ice

Hazardous Material: No

Storage

Storage Temperature: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

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