LDL Receptor Antibody

LDL Receptor Antibody

Product Information

Product Overview:

LDL Receptor (low-density lipoprotein receptor or LDLR) is a key determinant of plasma cholesterol levels and as a ubiquitously expressed cell membrane glycoprotein, it binds LDL, the major cholesterol-carrying lipoprotein of plasma, and transports it into cells via endocytosis. LDLR is found distributed from plasma membrane to intracellular compartments, cell surface (in the presence of PCSK9) and also localizes to Golgi apparatus as well as early/late endosomes and lysosomes. VLDL, IDL, HDL, and chylomicron remnant are also recognized by LDLR at neutral pH. Receptor-ligand complexes undergo endocytosis via clathrin-coated pits and coated vehicle dispenses to endosomes with LRP6 and ARH (also known as LDLR adaptor protein), connecting LDLR family protein and the endocytic machinery; thereby, acidic condition activates dissociation of internalized ligands. Released ligand particles further travel to lysosomes wherein the ligand is degraded by enzyme, while the receptors recycle back to cell surface. After internalization, LDL particles trigger reduction in HMGCR expression to suppress cholesterol biosynthesis; enhancement of ACAT activity to reduce toxic free cholesterol, and suppression of LDLR synthesis to reduce LDL uptake via SREBPs. Genetic mutations impairing LDLR function results in a condition with extremely elevated serum LDL levels and early onset atherosclerosis known as familial hypercholesterolemia (FH).

Application:

This LDL Receptor antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot, where bands are seen ~95 kDa and ~160 kDa representing the unglycosylated and glycosylated forms of the LDL receptor, respectively.

Catalog#: NBP1-06709
LID#: 171713A
Quantity :
Order by Phone or Fax: 1.800.316.3081

Specifications

Host SpeciesRabbit
SpeciesHuman, Mouse
ControlsHepG2 cell lysate, Antigen standard for LDLR (NM_000527) (transient overexpression lysate)
Entrez Human ID3949
LocalizationMembrane; Single-pass type I membrane protein. Clathrin-coated pit.
Preservation0.1% Sodium Azide
Species ReactivityHuman and Mouse. Predicted to react with monkey based on 100% sequence homology.
Swissprot Human IDP01130
Gene SymbolLDLR
ApplicationsWB, ICC/IF, IHC
Species AbbreviationHu, Mu
Grade/PurityImmunogen affinity purified
ClonalityPolyclonal
ImmunogenSynthetic peptide made to an internal portion of the human LDL Receptor protein (within residues 500-550). [Swiss-Prot# P01130]
SpecificityThis is specific for both the unglycosylated and glycosylated forms of the LDL Receptor.
Storage BufferPBS, 30% glycerol
DilutionImmunocytochemistry/Immunofluorescence 1:1000-1:10000, Immunohistochemistry, Western Blot 0.5 ug/ml
Gene ID10903
Unit Size0.1ml
Concentration1.41 mg/ml
Safety StatementsThis product is for research use only and is not approved for use in humans or in clinical diagnosis.
Sample AvailabilityYes
SynonymFHC, FH, LDL receptor, LDLCQ2, low density lipoprotein receptor, low-density lipoprotein receptor, low-density lipoprotein receptor class A domain-containing protein 3

Shipping & Handling

Estimated Ship Time: 3 Business Days

Shipped In: Blue Ice

Hazardous Material: No

Storage

Storage Temperature: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

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