LC3I Antibody [Biotin]

LC3I Antibody [Biotin]

Product Information

Product Overview:

LC3I (also known as LC3A, MAP1A, MAP1B, and Microtubule-associated protein 1 light chain 3 alpha) is one of the light chain components of the microtubule-associated proteins that regulate physical interactions between microtubules and portions of the cytoskeleton. LC3A also plays a role in the formation of autophagosomal vacuoles (autophagosomes). LC3I is mostly expressed in brain, liver, heart, skeletal muscle, and testis. Conversely, LC3I is not expressed in thymus, and has low expression in peripheral blood leukocytes which can be directly attributed to coronary artery disease (CAD) where autophagosome formation is decreased. The decrease in autophagosome formation is directly correlated to a decrease in LC3A expression and the autophagy of circulating leukocytes contributes to the pathogenesis of atherosclerosis and coronary artery disease (PMID: 21812771). Furthermore, LC3A and its role in autophagy, has been used in studies of tumor hypoxia and virology (PMID: 21537144, 21507968).

Application:

This LC3I antibody is useful for Western Blot, Immunocytochemistry/Immunofluorescence, and IHC-paraffin embedded sections. In Western Blot, a band is seen ~15kDa representing LC3I. In ICC/IF, observed staining showed inactivated LC3 throughout the cytoplasm of Neuro2a cells. In IHC-P, staining was observed in the cytoplasm of mouse testes tissue. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

Catalog#: NBP1-78964B
LID#: 171663A
Quantity :
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Specifications

Host SpeciesRabbit
SpeciesHuman, Mouse, Rat, Bovine, Primate, Porcine
Entrez Human ID84557
LocalizationCytoplasm.
PreservationNo Preservative
Species ReactivityHuman, mouse, rat, primate, porcine, and bovine.
Gene SymbolMAP1LC3A
ApplicationsWB, ICC/IF, IHC, IHC-P
Species AbbreviationHu, Mu, Rt, Bv, Mk, Po
Grade/PurityAffinity purified
ClonalityPolyclonal
ImmunogenA synthetic peptide made to a C-terminal region of the human LC3I protein (within residues 50-120). [Swiss-Prot Q9H492]
ConjugationBiotin
SpecificityThis LC3I antibody specifically detects the cytosolic form of LC3 before it gets converted into LC3II during autophagy.
Storage BufferPBS
DilutionWestern Blot 1:1000, Immunohistochemistry-Paraffin 1:400, Immunocytochemistry/Immunofluorescence 1:75, Immunohistochemistry 1:400
Unit Size0.1ml
Concentration1.09 mg/ml
Safety StatementsThis product is for research use only and is not approved for use in humans or in clinical diagnosis.
Sample AvailabilityYes
SynonymMAP1 light chain 3 like protein 1, MAP1 light chain 3-like protein 1, MAP1LC3A, Microtubule-associated proteins 1A/1B light chain 3A

Shipping & Handling

Estimated Ship Time: 3 Business Days

Shipped In: Blue Ice

Hazardous Material: No

Storage

Storage Temperature: Store at 4C in the dark.

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