LC3B Antibody [Biotin]

LC3B Antibody [Biotin]

Product Information

Product Overview:

Autophagy is a process of intracellular bulk degradation in which cytoplasmic components, including organelles, are sequestered within double-membrane vesicles that deliver the contents to the lysosome/vacuole for degradation. During macroautophagy, the sequestering vesicles, termed autophagosomes, fuse with the lysosome or vacuole resulting in the delivery of an inner vesicle (autophagic body) into the lumen of the degradative compartment. There are 16 proteins participating in the autophagy pathway in humans. The autophagy protein LC3, a mammalian homologue of Atg8, was originally identified as microtubule-associated protein 1 light chain 3. It is a component of both the MAP1A and MAP1B microtubule-binding domains and the heavy-chain independent regulation of LC3 expression may modify MAP1 microtubule-binding activity during development. LC3 is the only known mammalian protein identified that stably associates with the autophagosome membranes. LC3-I is cytosolic and LC3-II is membrane bound and enriched in the autophagic vacuole fraction. The detection of LC3-I to LC3-II conversion is a useful and sensitive marker for distinguishing autophagy in mammalian cells.

Application:

This LC3 antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry-frozen sections, Flow Cytometry and Western Blot where bands are seen ~17 and 19 kDa corresponding to LC3-II and LC3-I. Electron Microscopy was reported in scientific literature.

Catalog#: NB600-1384B
LID#: 171638A
Quantity :
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Specifications

Host SpeciesRabbit
SpeciesHuman, Mouse, Rat, Bovine, Canine, Primate, Zebrafish
Entrez Human ID81631
LocalizationLC3-I is cytoplasmic. LC3-II binds to the autophagic membranes.
MarkerAutophagosomes Marker
PreservationNo Preservative
Species ReactivityHuman, mouse, rat and bovine. Zebrafish reactivity reported in scientific literature (PMID: 23724125). Canine and primate reactivity reported in scientific literature (PMID: 24027311) Other species have not been tested.
Swissprot Human IDQ9GZQ8
Gene SymbolMAP1LC3B
ApplicationsWB, ICC/IF, IHC, IHC-P
Species AbbreviationHu, Mu, Rt, Bv, Ca, Mk, Ze
Grade/PurityImmunogen affinity purified
ClonalityPolyclonal
ImmunogenA synthetic peptide made to the N-terminal region of the human LC3B protein. [UniProt# Q9GZQ8]
ConjugationBiotin
Storage BufferPBS
DilutionImmunocytochemistry/Immunofluorescence 1:2000, Immunohistochemistry 1:2000, Immunohistochemistry-Paraffin 1:2000, Western Blot 1:3000
Gene ID5971
Unit Size0.1ml
Concentration1.00 mg/ml
Safety StatementsThis product is for research use only and is not approved for use in humans or in clinical diagnosis.
Sample AvailabilityYes
SynonymATG8F, Autophagy-related protein LC3 B, Autophagy-related ubiquitin-like modifier LC3 B, LC3B, lc3-i, ii, MAP1 light chain 3-like protein 2, MAP1A/1BLC3, MAP1A/MAP1B LC3 B, MAP1ALC3, microtubule-associated proteins 1A/1B light chain 3B, microtubule-associated proteins 1A/1B light chain 3, microtubule-associated protein 1 light chain 3 betaMAP1A/MAP1B light chain 3 B

Shipping & Handling

Estimated Ship Time: 3 Business Days

Shipped In: Blue Ice

Hazardous Material: No

Storage

Storage Temperature: Store at 4C in the dark.

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