LC3 Antibody [HRP]

LC3 Antibody [HRP]

Product Information

Product Overview:

LC3, a mammalian homologue of Apg8, was originally identified as microtubule-associated protein 1 light chain 3. It is a component of both the MAP1A and MAP1B microtubule-binding domains and the heavy-chain independent regulation of LC3 expression might modify MAP1 microtubule-binding activity during development. However, LC3 is now thought to also be involved in autophagy. LC3-I is cytosolic and LC3-II is membrane bound and enriched in the autophagic vacuole fraction. LC3-II is the first mammalian protein identified that specifically associates with the autophagosome membranes.

Application:

This LC3 antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry-paraffin embedded sections, Immunoprecipitation and Western Blot. Use in Immunohistochemistry-Frozen sections reported in scientific literature (PMID: 20008275) In Western blot bands are seen ~17 and 19 kDa corresponding to LC3-II and LC3-I. In some cases a non-specific band is seen at ~21 kDa in mouse protein. In ICC/IF, cytoplasmic staining was observed in HeLa cells.

Catalog#: NB100-2220H
LID#: 171630A
Quantity :
Order by Phone or Fax: 1.800.316.3081

Specifications

Host SpeciesRabbit
SpeciesHuman, Mouse, Rat, Bacteria, Porcine, Golden Syrian Hamster, Zebrafish
ControlsHuman/mouse brain lysate, ES, HeLa, HEK293, MCF7 cells, human lymphoblasts, and embryoid bodies, Antigen standard for MAP1LC3B (NM_022818) (transient overexpression lysate)
Entrez Human ID81631
LocalizationLC3-I is cytoplasmic. LC3-II binds to the autophagic membranes.
MarkerAutophagosomes Marker
PreservationNo Preservative
Species ReactivityHuman, rat, mouse, zebrafish, bacteria, hamster, and porcine. The mouse detection has been reported to be weaker than the human. Immunogen sequence has 91% homology to bovine and 84% homology to Xenopus.
Swissprot Human IDQ9GZQ8
Gene SymbolMAP1LC3B
ApplicationsWB, IP
Species AbbreviationHu, Mu, Rt, Ba, Po, SyHa, Ze
Grade/PurityImmunogen affinity purified
ClonalityPolyclonal
ImmunogenA synthetic peptide made to an N-terminal portion of the human LC3 protein sequence (between residues 1-100). [UniProt# Q9GZQ8]
ConjugationHRP
Storage BufferPBS
DilutionImmunoprecipitation 1:200-1:400, Western Blot 2 ug/ml
Gene ID10840
Unit Size0.1ml
Concentration1.0 mg/ml
Safety StatementsThis product is for research use only and is not approved for use in humans or in clinical diagnosis.
Sample AvailabilityYes
SynonymATG8E, Autophagy-related protein LC3 A, Autophagy-related ubiquitin-like modifier LC3 A, lc3-i, ii, LC3, LC3A, MAP1A/1B light chain 3 A, MAP1ALC3MAP1A/MAP1B LC3 A, MAP1BLC3MAP1A/MAP1B light chain 3 A, microtubule-associated proteins 1A/1B light chain 3A, microtubule-associated proteins 1A/1B light chain 3, microtubule-associated protein 1 light chain 3 alphaMAP1 light chain 3-like protein 1

Shipping & Handling

Estimated Ship Time: 3 Business Days

Shipped In: Blue Ice

Hazardous Material: No

Storage

Storage Temperature: Store at 4C in the dark.

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