Product Overview:
Many transcripts for proteins specifically expressed in haploid germ cells are synthesized in the spermatogenesis. A monoclonal antibody TRA54 recognizes specific organelles in germ cell cytoplasm from spermatocytes to spermatids. Western blots using TRA54 revealed broad bands with 85 kD, 190 kD and >200 kDa in testis. This antibody can be used for immunohistochemical staining of testicular tissues. The hybridoma producing TRA54 was obtained from the immunization of a rat with cell lysates of adult mouse testes by Prof. Yoshitake Nishimune group of Osaka University. This product is purified from the ascitics fluid of nude mice carrying the hybridoma clone TRA54.